LPAM-1 Rat anti-Mouse, Brilliant Violet 711, Clone: DATK32, BD Optibuild™

Rat Monoclonal Antibody

Supplier:  BD Biosciences 740701

Catalog No. BDB740701

Description

The DATK32 monoclonal antibody specifically binds to an epitope specific for the α 4β 7 integrin heterodimer. α 4β 7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells. It interacts with several ligands, including VCAM-1 (CD106), fibronectin, and MAdCAM-1. DATK32 antibody induces α 4β 7-dependent lymphocyte aggregation, but it inhibits other α

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

• Antigen: LPAM-1
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Itga4Itgb7; α4β7 Integrin; alpha 4 beta 7 integrin; CD49dβ7
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: DATK32
• Conjugate: Brilliant Violet 711
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: AKR/Cum mouse T lymphoma line TK1
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only