MilliporeSigma™ Rat anti-Progranulin/GRN, Clone: 8H10,

Catalog No.	Quantity
MABN1795MI	100 μg

Catalog No. MABN1795MI Supplier MilliporeSigma™ Supplier No. MABN1795

Description

Rat Monoclonal Antibody

Specifically detects Progranulin/GRN clone: 8H10 in Mouse samples, and it is validated for Immunohistochemistry, Western Blotting

Granulins constitute a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 602 aa murine precursor protein, preprogranulin (UniProt Q3U9N4), is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide (aa 1-17) produces progranulin (Acrogranin; aa 18-589), which can be further cleaved into 7 active peptides named granulin-1 through granulin-7. Both the peptides and progranulin protein regulate cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Heterozygous loss-of-function mutations in the GRN gene is a known cause for frontotemporal lobar degeneration (FTLD) with accumulation of TAR DNA-binding protein (TDP)-43, while a complete GRN deficiency due to a homozygous loss-of-function mutation causes the lysosomal storage disorder known as neuronal ceroid lipofuscinosis (NCL). In Grn-/- mice, a murine model for GRN-associated FTLD-TDP (FTLD-TDP/GRN), the lysosomal proteins cathepsin D (CTSD) is found to be elevated, while an upregulated granulin level is observed in Ctsd(-/-) mice, a model for NCL. Moreover, both types of knockout mice are found to display increased levels of transmembrane protein 106B (TMEM106B) as a common phenotype.

Specifications

• Antigen: Progranulin/GRN
• Applications: Immunohistochemistry, Western Blot
• Classification: Monoclonal
• Clone: 8H10
• Formulation: Purified rat monoclonal IgG2cκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: Q3U9N4
• Gene Symbols: Grn
• Host Species: Rat
• Immunogen: Linear peptide corresponding to rat progranulin sequence near the C-terminus
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_032201
• Target Species: Mouse
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Form: Purified
• Isotype: IgG2c κ

For Research Use Only