MilliporeSigma™ Rat anti-Semaphorin 4D/CD100, Clone: BMA-12,

Rat Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC987

Catalog No. MABC987MI

Description

Specifically detects Semaphorin 4D/CD100 clone: BMA-12 in Mouse samples, and it is validated for Activity Assay, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blotting

Semaphorin 4D (alson known as CD100, Sema J, M-sema G, Semaphorin H, Semaphorin-J, Semaphorin-C-like 2) is a single-transmembrane protein encoded by the Sema4d gene in mouse. Semaphorins are involved in a wide range of biological processes, including axon guidance, neuronal migration, angiogenesis, cardio- and osteo-genesis, via interaction with a family of transmembrane proteins called Plexins expressed on other cells. Semaphroins and Plexins interact via their conserved sema domains, each sema domain forms a seven-blade beta-propeller fold in the extracellular domain of the respective protein. Semaphorin 4D extracellular domain can also be cleaved off and function in soluble form. Estrogen-stimulated Sema4D shedding is important for apoptosis induction of Plexin-B1-expressing vaginal epithelium during postnatal tissue remodeling (PMID 24841081). In addition, MT1-MMP-mediated Sema4D shedding is reported to induce endothelial cell chemotaxis in vitro and blood vessel growth in vivo and forms the basis of tumor-induced angiogenesis among several head and neck squamous cell carcinoma cell lines (PMID 17204469). Interference of cell communication between Sema4D-expressing osteoclasts and PlexinB1-expressing osteoblasts is reported to be therapeutically beneficial in a murine model of osteoporosis (PMID 25088728 & 25252878).

Specifications

• Antigen: Semaphorin 4D/CD100
• Classification: Monoclonal
• Formulation: Purified rat monoclonal IgG2a in buffer containing PBS without preservatives.
• Gene Symbols: Sema4d; Semacl2; Semaj
• Immunogen: Recombinant protein corresponding to mouse Semaphorin 4D/CD100.
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_001268809
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Isotype: IgG2a

• Applications: Functional Assay, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Clone: BMA-12
• Gene Accession No.: O09126
• Host Species: Rat
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Form: Purified

For Research Use Only