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Specifications
Specifications
| Preparation Method | Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume / lane is 10-20 μL depending on the size format of your gel. |
| Quantity | 500 μg |
| Purity | Multi-step |
| Host Species | Rat |
| Content And Storage | Store at -70°C. Avoid freeze/thaw cycles. |
| Target | Brain |
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