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Invitrogen™ eBioscience™ Rat IgG1 ELISA Ready-SET-Go!™ Kit Non-distribution product as customer accommodation.

For quantitative detection of rat IgG1.

Manufacturer:  Invitrogen™ 885050022

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Catalog No. 50-112-5131


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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Rat IgG1 isotype control (standard), lyophilized, 800ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)

Description

Description

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

6.25-400 ng/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Plasma,Serum,Supernatant
Plasma,10 μL|Serum,10 μL|Supernatant,50 μL
2-8°C
24 hr 30 min
Immunoglobulin G
HRP
96-well plates
10X Coating Buffer: Buffer for plating the Capture Antibody
2 x 96 tests
6.25 ng/mL
20μL (prediluted 1:10,000)
Rat
1 hr 45 min
Rat
HRP
Documents
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