Gibco™ Rat IL-15 Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Rat IL-15 is a 13.5 kDa protein consisting of 115 amino acid residues. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

IL-15 (Interleukin 15 ) is a cytokine that regulates T and natural killer cell activation and proliferation. IL-15 and IL-2 share many biological activities as both have been found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between IL-15 and IL-2. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. In mouse, studies suggest that IL-15 may increase the expression of apoptosis inhibitor Bcl-xL, possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. As such, IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases. Further, IL-15 is a widely expressed pro-inflammatory cytokine and has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes.

Specifications

• Accession Number: P97604
• For Use With (Application): Functional Assay
• Formulation: protein with no preservative
• Gene ID (Entrez): 25670
• Molecular Weight (g/mol): 13.5 kDa
• Name: Rat IL-15
• Quantity: 100 μg
• Source: E. coli
• Regulatory Status: RUO
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: AI503618; il 15; Il15; IL-15; ILN; Interleukin; interleukin 15; Interleukin15; interleukin-15; MGC9721; M-IL-15
• Common Name: IL-15
• Gene Symbol: IL15
• Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of murine CTLL-2 cells is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 10^5 units/mg
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MNWIDVRYDL EKIESLIQFI HIDTTLYTDS DFHPSCKVTA MNCFLLELQV ILHEYSNMTL NETVRNVLYL ANSTLSSNKN VIESGCKECE ELEERNFTEF LQSFIHIVQM FINTS
• Content And Storage: -20°C
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.