Invitrogen™ Rat IL-18 Recombinant Protein

Catalog No.	Quantity
ENRRIL18I	20 μg

Catalog No. ENRRIL18I Supplier Invitrogen™ Supplier No. RRIL18I

Description

Recombinant Protein

Mature rat IL-18 was expressed in Pichia pastoris, X-33. GenBank™ Accession # P97636, amino acids 37 - 194. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HFGRLHCTTA. Purity is >95% by SDS-PAGE analyses. Activity: The biological activity of recombinant rat IL-18 was determined by its ability to induce IFNg production from KG-1 cells in the presence of recombinant mouse IL-12. 480 pg/mL and 610 pg/mL of IFNg were induced by 20 ng/mL and 40 ng/mL of recombinant rat IL-18, respectively. Use: For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments. Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 0.5 mL of distilled or deionized water. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

The protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein can specifically interact with this cytokine, and thus negatively regulate its biological activity.

Specifications

• Accession Number: P97636
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: PBS with no preservative
• Gene ID (Entrez): 29197
• Molecular Weight (g/mol): 18.3 kDa
• Name: Rat IL-18
• Quantity: 20 μg
• Source: Yeast
• Regulatory Status: RUO
• Gene Alias: cytokine; iboctadekin; IFN-gamma-inducing factor; IGIF; il 18; IL-1 gamma; IL18; IL-18; IL1F4; IL-1 g; ILN; interferon gamma inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Interleukin; interleukin 18; interleukin 18 (interferon-gamma-inducing factor); interleukin-1 gamma; Interleukin18; Interleukin-18; interleukin-18 proprotein; interleukin-18-like protein; MGC12320
• Common Name: IL-18
• Gene Symbol: Il18
• Biological Activity: ED50 = 20 - 40 ng/mL; determined by the dose-dependent induction of IFN-gamma production from KG-1 cells in the presence or recombinant mouse IL-12.
• Conjugate: Unconjugated
• Species: Yeast
• Recombinant: Recombinant
• Sequence: Rat IL-18, amino acids 37-194 (Accession # P97636)
• Content And Storage: 4°C
• Expression System: P. pastoris
• Form: Lyophilized
• Protein Subtype: Recombinant
• Purity or Quality Grade: >95% by SDS-PAGE