Invitrogen™ Rat CCL2 (MCP-1) Recombinant Protein

Description

Recombinant Protein

RRMCP110 contains 125 amino acid residues. Endotoxin level is <1 EU/ug. Purity: >97% pure by SDS-PAGE and HPLC analyses. Activity: The maximal chemotactic activity on human monocytes is achieved at 100 ng/mL. Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 μg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V). Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Specifications

• Accession Number: P14844
• For Use With (Application): Control, ELISA, Immunoprecipitation, Western Blot
• Formulation: protein with no preservative
• Gene ID (Entrez): 24770
• Molecular Weight (g/mol): 14 kDa
• Name: Rat CCL2 (MCP-1)
• Quantity: 10 μg
• Endotoxin Concentration: <1 EU/ μg
• Gene Alias: Acidic seminal fluid protein; AI323594; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; Je; MCAF; Mcp1; MCP-1; MCP1A; MCP-1 A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; monocyte chemotactic protein 1 A; Monocyte secretory protein JE; monocyte-chemoattractant protein-1 precursor; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; SCYA2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; SMC-CF
• Common Name: CCL2 (MCP-1)
• Gene Symbol: CCL2
• Biological Activity: Maximal chemotactic activity on human monocytes is achieved at 100 ng/mL
• Conjugate: Unconjugated
• Species: E. coli
• Recombinant: Recombinant
• Sequence: Rat CCL2 recombinant protein contains 125 amino acids
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: >97% by SDS-PAGE and HPLC analyses