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Invitrogen™ Rat VEGFC ELISA Kit

Catalog No. p-7092128
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Quantity:
10 x 96 Tests
96 Tests

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat VEGF-C
Biotin-Conjugate anti-rat VEGF-C monoclonal antibody
Streptavidin-HRP
Rat VEGF-C Standard lyophilized, 6ng/mL upon reconstitution
Sample Diluent
Conjugate Diluent
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Rat Vascular Endothelial Growth Factor C (Rt VEGFC) ELISA quantitates Rt VEGFC in rat serum or cell culture medium. The assay will exclusively recognize both natural and recombinant rat VEGFC. Principle of the method The Rat VEGFC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis and endothelial cell growth, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O35757
Assay Range 47.0 to 3,000 pg/mL
Assay Sensitivity 27.0 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Rat
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 114111
Gene Symbol Vegfc
Interassay CV 2.7%
Intraassay CV 5.1
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Synonym VRP, FLT-4 ligand
Target VEGFC
Test Time 1 hrs 20 mins
Total Assay Time 3 hrs 30 mins
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