Invitrogen™ Rb (Cleaved DRb-p70) Monoclonal Antibody (172C1094)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA141186

Catalog No. 01-676-588

Description

Suggested positive control: camptothecin-treated HL-60 (24 hr).

The retinoblastoma gene product (Rb) is a tumor suppressor protein and acts as cell cycle regulator (1). Recently, Rb has been shown to play a role in apoptosis. During apoptosis, Rb is subjected to cleavage by caspase-3 like protease. Caspase-3 cleaves Rb into 48 and 68kDa fragments (2-4). Cleavage of Rb would make it functionally inactive, causing release of E2F-1. The E2F-1 then may promote apoptosis (5).

Specifications

• Antigen: Rb (Cleaved DRb-p70)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.05% BSA and 0.05% sodium azide
• Gene Accession No.: P06400, P13405
• Gene Symbols: RB1
• Immunogen: Caspase-3-induced cleavage site of human Rb.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Clone: 172C1094
• Conjugate: Unconjugated
• Gene: RB1
• Gene Alias: Cleaved Rb; delta RB-p70; DRb-p70; exon 17 tumor GOS561 substitution mutation causes premature stop; GOS563 exon 17 substitution mutation causes premature stop; OSRC; p105-Rb; pp105; pp110; PPP1R130; pRb; prepro-retinoblastoma-associated protein; protein phosphatase 1, regulatory subunit 130; Rb; RB transcriptional corepressor 1; RB1; Rb-1; Rb-p70; retinoblastoma 1; retinoblastoma suspectibility protein; retinoblastoma-associated protein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 19645, 5925
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov