RB1 Antibody (172C1094), Alexa Fluor™ 750 - (cleaved), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NB10056598AF750

Catalog No. N10698A70

Description

RB1 Monoclonal specifically detects RB1 in Human, Mouse samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Knockdown Validated.

Specifications

• Antigen: RB1
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50 mM sodium borate with 0.05% sodium azide
• Gene Symbols: RB1
• Immunogen: A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen.
• Primary or Secondary: Primary
• Test Specificity: The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ∼68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).
• Content And Storage: Store at 4°C in the dark.

• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), KnockDown
• Clone: 172C1094
• Dilution: Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Knockdown Validated
• Gene Alias: OSRC, osteosarcoma, p105-Rb, pp110, pRb, Rb, retinoblastoma 1, retinoblastoma suspectibility protein, retinoblastoma-associated protein
• Host Species: Mouse
• Quantity: 0.1 mL
• Gene ID (Entrez): 5925
• Target Species: Human, Mouse
• Isotype: IgG1