RB1 Antibody (172C1094) - (cleaved), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Catalog Number	Mfr. No.	Quantity
NB10056598T	Novus Biologicals NB10056598SS	0.025 mg
NB10056598	Novus Biologicals NB10056598	0.1 mg

Description

RB1 Monoclonal specifically detects RB1 in Human, Mouse samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Knockdown Validated.

Specifications

• Antigen: cleaved Retinoblastoma 1
• Concentration: 0.5 mg/mL
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Cancer, Cell Cycle and Replication, Transcription Factors and Regulators, Tumor Suppressors
• Gene Accession No.: Q03137
• Gene ID (Entrez): 5925
• Immunogen: A synthetic peptide corresponding to a region of the caspase-3-induced cleavage site of human Rb was used as immunogen.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: 172C1094
• Dilution: Western Blot 2 ug/ml, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:10-1:500, Knockdown Validated
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Gene Alias: OSRC, osteosarcoma, p105-Rb, pp110, pRb, Rb, retinoblastoma 1, retinoblastoma suspectibility protein, retinoblastoma-associated protein
• Gene Symbols: RB1
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ∼68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).

For Research Use Only