Invitrogen™ RbAp48 Monoclonal Antibody (11G10)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA123273

Catalog No. MA123273

Description

Positive control: MOLT4, HeLa, LB22. For ICC a permeabilization step is recommended; use at a dilution of 1:500-1:1000 in PBST containing 2%BSA and 10% normal serum at 4°C overnight. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Specifications

• Antigen: RbAp48
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q09028, Q60972
• Gene Symbols: RBBP4
• Immunogen: The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ChIP Assay, Functional Assay, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: 11G10
• Conjugate: Unconjugated
• Gene: RBBP4
• Gene Alias: CAF-1 p48 subunit; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; histone-binding protein RBBP4; lin-53; mRbAp48; MSI1 protein homolog; nucleosome-remodeling factor subunit RBAP48; NURF55; RB binding protein 4, chromatin remodeling factor; RBAP48; RBBP4; RBBP-4; retinoblastoma binding protein 4; retinoblastoma-binding protein 4; Retinoblastoma-binding protein p48
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 19646, 313048, 5928
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid