Invitrogen™ RBM25 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA590511

Catalog No. PIPA590511

Description

Immunogen sequence: KPTLRPISSA PSVSSASGNA TPNTPGDESP CGIIIPHENS PDQQQPEEHR PKIGLSLKLG ASNSPGQPNS VKRKKLPVDS VFNKFEDEDS DDVPRKRKLV PLDYGEDDKN ATKGTVNTEE KRKHIKSLIE KIPTAKPELF AYPLDWSIVD SILMERRIRP WINKKIIEYI GEEEATLVDF VCSKVMAHSS PQSILDDVAM VLDEEAEVFI VKMWRLLIYE TEAKKIGLVK; Positive Samples: DU145, OVCAR3, HepG2, Jurkat, NIH/3T3, mouse brain, mouse liver; Cellular Location: Cytoplasm, Nucleus speckle.

RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.

Specifications

• Antigen: RBM25
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RBM25
• Gene Alias: 2600011C06Rik; 2610015J01Rik; A130095G20Rik; AI159652; AL023075; arg/Glu/Asp-rich protein of 120 kDa; Arg/Glu/Asp-rich protein, 120 kDa; AU043498; fSAP94; functional spliceosome-associated protein 94; LOC540258 protein; NET52; Protein S164; Rbm25; RED120; RNA binding motif protein 25; rna-binding; RNA-binding motif protein 25; RNA-binding protein 25; RNA-binding protein 25-like protein; RNA-binding region (RNP1, RRM) containing 7; RNA-binding region-containing protein 7; RNPC7; S164; Snu71; U1 small nuclear ribonucleoprotein 1SNRP homolog
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 58517, 67039
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 0.87 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: B2RY56, P49756
• Gene Symbols: RBM25
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 614-843 of human RBM25 (NP_0670621).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG