R&D Systems™ Recombinant Canine Tie-2 His-tag Protein

Description

Recombinant Protein

Measured by its binding ability in a functional ELISA. When Recombinant Canine Tie-2 His-tag (Catalog # 10781-T2) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Angiopoietin-2 (Catalog # 623-AN ) binds with an ED50 of 0.40-3.60 ng/mL

Specifications

• Accession Number: XP_005626754.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 7010
• Molecular Weight (g/mol): M.W.-observed: 95-105 kDa, under reducing conditions, M.W.-theroretical: 82 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived canine Tie-2 protein Ala23-Lys746, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK, TEK tyrosine kinase, endothelial, Tie2, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Tie-2