Novus Biologicals™ Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase His Protein

Description

Highly purified and high bioactivity. Generating reliable and reproducible results.

• A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-168 of E.coli Double-Stranded Uracil-DNA Glycosylase
• G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family.
• It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear.
• The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site.
• It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base.
• The complementary strand guanine functions in substrate recognition.
• Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Specifications

• Concentration: 0.5 mg/mL
• For Use With (Application): SDS-PAGE
• Formulation: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol
• Molecular Weight (g/mol): 21.1 kDa
• Quantity: 50 μg
• Source: E. coli
• Storage Requirements: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Gene Alias: double-strand uracil-DNA glycosylase, Double-strand-specific uracil glycosylase, dsDNA specific UDG, DsUDG, EC 3.2.2.28, G/U mismatch-specific DNA glycosylase, G:T/U mismatch-specific DNA glycosylase, Mismatch-specific uracil DNA glycosylase, Mug, Xanthine DNA glycosylase
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol
• Purity or Quality Grade: >90%, by SDS-PAGE