R&D Systems™ Recombinant Human ACE-2 Fc Chimera Protein

Description

Bioactive, High Spike RBD Binding

Measured by its binding ability in a functional ELISA with Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV ). Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is >550 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q9BYF1.2
• For Use With (Application): Bioactivity, Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
• Gene ID (Entrez): 59272
• Molecular Weight (g/mol): M.W.-observed: 124-130 kDa, under reducing conditions, M.W.-theoretical: 110 kDa
• Quantity: 20 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human ACE-2 protein Human ACE-2 (Gln18-Ser740) (N-terminus) Accession # Q9BYF1.2 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: ACE2, ACEH, ACEHangiotensin I converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: ACE-2