R&D Systems™ Recombinant Human Angiostatin Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by the ability of the immobilized protein to support the adhesion of THP-1 human acute monocytic leukemia cells. Chavakis, T. et al. (2005) Blood 105:1036. The ED50 for this effect is 0.5-2.5 μg/mL.

Specifications

• Accession Number: P00747
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 5340
• Molecular Weight (g/mol): M.W. (Observed): 75-85 kDa, reducing conditions; M.W. (theoretical): 67.7 kDa (monomer)
• Name: Angiostatin Fc Chimera
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Angiostatin protein Met-Asp Human IgG1 (Pro100-Lys330) IEGR Human Angiostatin (Kringle 1-4) (Val98-Ala459) (N-terminus) Accession # P00747 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: PLG
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining, under reducing conditions
• Protein: Angiostatin