R&D Systems™ Recombinant Human Asparagine Synthetase/ASNS His Protein

Description

Asparagine Synthetase (ASNS) represents a single mammalian-expressed enzyme that catalyzes the synthesis of asparagine and glutamate from aspartate and glutamine in an ATP-dependent amidotransferase reaction

• Although the name focuses attention on its function in asparagine synthesis, the reaction catalyzed by ASNS also impacts glutamine, aspartate and glutamate homeostasis.
• Although ubiquitously expressed at low levels in many organs, expression is particularly high in the pancreas.

Specifications

• Accession Number: P08243-1
• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 440
• Molecular Weight (g/mol): 65 kDa
• Purification Method: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Quantity: 20 μg
• Source: Human embryonic kidney cell, HEK293-derived human Asparagine Synthetase/ASNS protein Cys2-Ala561 with C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP and Glycerol