R&D Systems™ Recombinant Human B7-1/CD80 Fc Chimera Protein

Description

Disulfide-linked homodimer

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.025-0.15 μg/mL in the presence of PHA.

Specifications

• Accession Number: P33681
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 941
• Molecular Weight (g/mol): M.W. (Observed): 66-80 kDa, under reducing conditions; M.W. (theoretical): 50.5 kDa
• Purification Method: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Quantity: 100 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human B7-1/CD80 protein Human B7-1/CD80 (Val35-Asn242) (N-terminus) Accession # P33681 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: B7-1/CD80