R&D Systems™ Recombinant Human C1GalT1/C1GalT1C1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to transfer galactose from UDP-galactose to 4-nitrophenyl-alpha-D-galactosaminide. The specific activity is >2,750 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q9NS00 (C1GalT1) & Q96EU7 (C1GalT1C1)
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 56913
• Molecular Weight (g/mol): M.W. (Observed): 25-42 kDa, reducing conditions; M.W. (theoretical): 38 kDa (C1GalT1) & 35 kDa (C1GalT1C1)
• Quantity: 20 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human C1GalT1 protein Asp45-Pro363, with N-terminal 6-His tag (C1GalT1); Ile28-Asp318, with an N-terminal HA tag (C1GalT1C1)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B3Gal-T8, Beta-1,3-galactosyltransferase, C1GALT, C1GalT1, C1GalT2, C38H2-L1, Core 1 beta1,3-galactosyltransferase 1, Core 1 Beta3-Gal-T1, Core 1 O-Glycan T-Synthase, core 1 synthase, glycoprotein-N-acetylgalactosamine3-beta-galactosyltransferase, 1, Core 1 UDP-galactose:N-acetylgalactosamine-alpha-R beta1,3-galactosyltransferase 1, COSMC, EC 2.4.1.122, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1, MST143, TNPS, T-synthase
• Common Name: C1GALT1
• Gene Symbol: C1GALT1
• Biological Activity: Measured by its ability to transfer galactose from UDP-galactose to 4-nitrophenyl-alpha-D-galactosaminide. The specific activity is >2,750 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Asp45-Pro363, with N-terminal 6-His tag (C1GalT1); Ile28-Asp318, with an N-terminal HA tag (C1GalT1C1)
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: C1GALT1