R&D Systems™ Recombinant Human CD117/c-kit His-tag Avi-tag Protein

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human SCF (Catalog # 7644-SC ) is coated at 1.00 μg/mL, 100 μL/well, Biotinylated Recombinant Human CD117/c-kit His-tag Avi-tag binds with an ED50 of 0.600-3.60 μg/mL.

Specifications

• Accession Number: P10721.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3815
• Molecular Weight (g/mol): MolecularWeight-observed: 85-105 kDa, under reducing conditions.; MolecularWeight-theroretical: 58 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD117/c-kit protein Human CD117/c-kit (Gln26-Thr520) (N-terminus) Accession # P10721.1 HHHHHH Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD117, CD117 antigen, ckit, C-Kit, EC 2.7.10, EC 2.7.10.1, KIT, PBT, piebald trait, Proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, SCF R, SCFR, SCFRmast/stem cell growth factor receptor, soluble KIT variant 1, Tyrosine-protein kinase Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD117/c-kit