R&D Systems™ Recombinant Human (DYKDDDDK) Ubiquitin Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Recombinant Human (DYKDDDDK) Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human (DYKDDDDK) Ubiquitin concentration of 0.5-1 mM.

Specifications

• Accession Number: P0CG47.1
• Formulation: Lyophilized from a solution in deionized water.
• Gene ID (Entrez): 7314
• Molecular Weight (g/mol): 9.8 kDa
• Name: FLAG Ubiquitin
• Quantity: 1mg
• Source: E. coli-derived human Ubiquitin protein Met1 - Gly76 with a N-terminal (DYKDDDDK) tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.