R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF (146 aa) Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.

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Specifications

• Accession Number: P09038
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in Tris-HCl and NaCl.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): 16.5kDa
• Quantity: 500 μg
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Pro143-Ser288,with an N-terminal Ala
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10EU per 1μg of the protein by the LAL method.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Gene Symbol: FGF2
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 to 250μg/mL in sterile PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.