R&D Systems™ Recombinant Human GM-CSF (CHO-expressed) Protein

Description

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 6 to 30 pg/mL.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 6-30 pg/mL.

Specifications

• Accession Number: P04141
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 1437
• Molecular Weight (g/mol): M.W. (Observed): 20-35 kDa, reducing conditions; M.W. (theoretical): 14.5 kDa
• Name: colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, CSF2, CSF-2, GMCSF, GM-CSF, GMCSFgranulocyte-macrophage colony-stimulating factor, granulocyte-macrophage colony stimulating factor, MGC131935, MGC138897, Molgramostim, molgramostin, sargramostim
• Quantity: 100 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human GM-CSF protein Ala18-Glu144
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: CSF2
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: GM-CSF