R&D Systems™ Recombinant Human ICOS (aa 21-134) His-tag Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) at 0.5 μg/mL (100 μL/well) can bind Recombinant Human ICOS with an ED50 of 25-125 ng/mL.

Specifications

• Accession Number: Q9Y6W8-1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 29851
• Molecular Weight (g/mol): M.W. (Observed): 20-34 kDa, reducing conditions; M.W. (theoretical): 14 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human ICOS protein Human ICOS (Glu21-Gln134) (N-terminus) Accession # Q9Y6W8-1 HHHHHH IEGR (C-terminus)
• Storage Requirements: 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Cross Reactivity: ICOS
• Species: Human
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: ICOS