R&D Systems™ Recombinant Human IL-1 beta/IL-1F2 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is <12 pg/mL. The specific activity of recombinant human IL-1 beta is approximately 1.4 x 105 IU/μg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680). Specific activity is for reference purposes only and is not routinely tested.

Specifications

• Accession Number: NP_000567
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. 1 mg pack size (01M) is supplied as a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Gene ID (Entrez): 3553
• Molecular Weight (g/mol): 17 kDa
• Quantity: 500 μg
• Source: E. coli-derived human IL-1 beta/IL-1F2 protein Ala117-Ser269
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. The 1 mg pack size (01M) can be stored for 1 month at 2 to 8 °C under sterile conditions after opening.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: catabolin, IL-1, IL1 beta, IL-1 beta, IL1B, IL-1B, IL1-BETA, IL-1F2, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
• Gene Symbol: IL1B
• Specific Reactivity: IL-1 beta/IL-1F2
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 50-200 μg/mL in sterile PBS.
• Buffer: Lyophilized from a 0.2μm filtered solution in PBS with Trehalose.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.