R&D Systems™ Recombinant Human IL-18/IL-1F4 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human IL-18/IL-1F4 Protein is derived from E. coli. The Recombinant Human IL-18/IL-1F4 Protein has been validated for the following applications: Bioactivity.

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Specifications

• Accession Number: Q14116
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3606
• Molecular Weight (g/mol): Predicted MW: 18kDa Observed MW: 18kDa, reducing conditions
• Quantity: 10 μg
• Source: E. coli-derived human IL-18/IL-1F4 protein Tyr37-Asp193
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: Iboctadekin, IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL18, IL-18MGC12320, IL-1F4, IL1F4iboctadekin, IL-1g, Interferon gamma-inducing factor, interleukin 18 (interferon-gamma-inducing factor), Interleukin-1 gamma, interleukin-18
• Common Name: IL-18/IL-1F4
• Gene Symbol: IL18
• Biological Activity: Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha . The ED50 for this effect is typically 1.5-9ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Tyr37-Asp193
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining