R&D Systems™ Recombinant Human IL-4 (CHO-expressed) Protein

Description

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. ED50 for this effect is 0.05 to 0.2 ng/mL.

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.05-0.2 ng/mL.

Specifications

• Accession Number: P05112
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3565
• Molecular Weight (g/mol): M.W. (Observed): 17-25 kDa, reducing conditions; M.W. (theoretical): 15.0 kDa
• Name: B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4B_cell stimulatory factor 1, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
• Quantity: 100 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-4 protein His25-Ser153
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B cell growth factor 1, BCDF, B-cell stimulatory factor 1, BCGF1, BCGF-1, binetrakin, BSF1, BSF-1, IL4, IL-4B_cell stimulatory factor 1, IL4E12, interleukin 4, interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
• Gene Symbol: IL4
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: IL-4