R&D Systems™ Recombinant Human IL-7 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL. The specific activity of Recombinant Human IL-7 is approximately 4.4 x 105 IU/μg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530). Specific activity is for reference purposes only and is not routinely tested.

Specifications

• Accession Number: P13232
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3574
• Molecular Weight (g/mol): 17 kDa
• Quantity: 50 μg
• Source: <EM>E. coli</EM>-derived human IL-7 protein Asp26-His177, with an N-terminal Met
• Storage Requirements: Manual defrost freezer; Avoid repeated freeze-thaw cycles; 12 mo. from receipt, -20 to -70°C as supplied; 1 mo, 2 to 8°C under sterile conditions after reconstitution; 3 mo, -20 to -70°C under sterile conditions after reconstitution
• Regulatory Status: RUO
• Endotoxin Concentration: <0.01 EU per 1 μg of the protein by the LAL method.
• Gene Symbol: IL7
• Conjugate: Unconjugated
• Cross Reactivity: IL-7
• Species: Human
• Reconstitution: Reconstitute 5 μg vials at 50 μg/mL in sterile PBS. Reconstitute 10 μg or larger vials (at 100 μg/mL in sterile PBS.)
• Buffer: Lyophilized from a 0.2μm filtered solution in PBS.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.