R&D Systems™ Recombinant Human M-CSF R/CD115 Fc Avi-tag Protein

Description

Biotinylated

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human M-CSF (Catalog # 216-MC ) is immobilized at 1 μg/mL (100 μL/well), it binds to Biotinylated Recombinant Human M-CSF R/CD115 Fc Chimera Avi-tag (Catalog # AVI10339) with an ED50 of 6-36 ng/mL.

Specifications

• Accession Number: P07333-1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 1436
• Molecular Weight (g/mol): Observed: 100-120 kDa, under reducing conditions; Theroretical: 83 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human M-CSF R/CD115 protein Human M-CSF R/CD115 (Ile20-Thr516) Accession # P07333.2(N-terminus) IEGRMD Human IgG1 (Pro100-Lys330) Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: M-CSFR/CD115