Novus Biologicals™ Recombinant Human Noggin Protein

Description

Recombinant Protein

Biological activity measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in PBS (pH 7.4), 0.02% Tween-20.
• Gene ID (Entrez): 9241
• Molecular Weight (g/mol): MolecularWeight-theroretical: 23 kDa
• Quantity: 5 μg
• Storage Requirements: Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.)
• Endotoxin Concentration: Less than 0.1 EU/μg of Noggin as determined by LAL method.
• Gene Alias: noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1
• Gene Symbol: NOG
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL Stock solutions should be apportioned into working aliquots and stored (at -20°C.)
• Form: Purified
• Research Category: Biologically Active Proteins, Neuroscience, Stem Cell Markers, Stem Cell Signaling Pathway
• Purity or Quality Grade: >95%, by SDS-PAGE and HPLC
• Protein: Noggin