R&D Systems™ Recombinant Human Noggin Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.0200-0.160 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP ).

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Specifications

• Accession Number: Q13253
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 9241
• Molecular Weight (g/mol): 23kDa (monomer)
• Quantity: 1 mg
• Source: Mouse myeloma cell line,NS0-derived human Noggin protein Gln28-Cys232
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10EU per 1μg of the protein by the LAL method.
• Gene Alias: NOG, noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A
• Gene Symbol: NOG
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 250μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.