Novus Biologicals™ Recombinant Human PDGF-AA Protein

Description

Recombinant Protein

Biological activity measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for tis effect is 50-200 ng/mL in a fluorometric assay using a redox sensitive dye.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
• Gene ID (Entrez): 5154
• Molecular Weight (g/mol): MolecularWeight-theroretical: 28.8 kDa
• Quantity: 100 μg
• Storage Requirements: Store at -20°C to -70°C as supplied. After reconstitution, store at 2 to 8 C for 1 month and (at -20°C to -70°C for long term storage. Avoid repeated freeze/thaw cycles.)
• Endotoxin Concentration: Less than 0.1 EU/μg of PDGF-AA as determined by LAL method.
• Gene Alias: PDGF1, PDGF-A
• Gene Symbol: PDGFA
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL Stock solutions should be apportioned into working aliquots and stored (at -20°C.)
• Form: Purified
• Research Category: Biologically Active Proteins
• Purity or Quality Grade: >95%, by SDS-PAGE
• Protein: PDGF-AA