R&D Systems™ Recombinant Human Semaphorin 3F Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Human Neuropilin-2 Fc Chimera (Catalog # 2215-N2 ) is immobilized at 2 μg/mL, 100 μL/well, it binds Recombinant Human Semaphorin 3F Fc Chimera. The concentration of Recombinant Human Semaphorin 3F Fc Chimera that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.

Specifications

• Accession Number: Q13275-1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
• Gene ID (Entrez): 6405
• Molecular Weight (g/mol): M.W. (Observed): 91-125 kDa, reducing conditions; M.W. (theoretical): 111 kDa
• Quantity: 25 μg
• Source: Mouse myeloma cell line, NS0-derived human Semaphorin 3 F protein Human Semaphorin 3 F (Ser19-Gln772) (Leu503Met, Arg585Ala, Arg586Ala, Arg651Ala) (N-terminus) Accession # Q13275-1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Cross Reactivity: Semaphorin 3F
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL aq. soln..
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Semaphorin 3 F