R&D Systems™ Recombinant Human Siglec-3/CD33 Fc Avi-Tag Protein

Description

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells.

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is 0.2-1.4 μg/mL. Measured by its binding ability in a functional ELISA. When Human Siglec-3/CD33 (6C5/2) Antibody (Catalog # MAB1137 ) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human Siglec-3/CD33 Fc Chimera (Catalog # AVI10467) binds with an ED50 of 0.15-1.2 μg/mL.

Specifications

• Accession Number: P20138.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 945
• Molecular Weight (g/mol): M.W. (Observed): 67-78 kDa, under reducing conditions; M.W. (theoretical): 15 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Siglec-3/CD33 protein Human Siglec-3/CD33 (Met17-His259) (N-terminus) Accession # P20138.2 IEGRMD Human IgG1 (Pro100-Lys330) Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Siglec-3/CD33