R&D Systems™ Recombinant Human Sonic Hedgehog/Shh (C24II) Animal-Free

Description

Also available, GMP Shh Protein, Catalog # 1845-GMP! Animal-free and manufactured under GMP guidelines for therapeutic manufacturing applications.

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 μg/mL.

Specifications

• Accession Number: NP_000184.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Gene ID (Entrez): 6469
• Molecular Weight (g/mol): M.W.-observed: 22 kDa, reducing conditions, M.W.-theroretical: 20 kDa
• Quantity: 500 μg
• Source: E. coli-derived human Sonic Hedgehog/Shh protein Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-200 μg/mL in PBS, and allow up to 24 hours (at 2°C to 8°C for complete reconstitution.)
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Sonic Hedgehog/Shh