R&D Systems™ Recombinant Human Thrombopoietin (NS0-expressed) Protein

Description

A new economical version of TPO made in E. coli is now available.

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 5-15 ng/mL.

Specifications

• Accession Number: P40225
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 7066
• Molecular Weight (g/mol): M.W. (Observed): 80-90 kDa, reducing conditions; M.W. (theoretical): 35 kDa
• Name: Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived human Thrombopoietin/Tpo protein Ser22-Gly353
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: THPO
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.1 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Thrombopoietin/THPO