R&D Systems™ Recombinant Human TNF-alpha (HEK293-expressed) Protein

Description

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. ED50 for this effect is 20 to 100 pg/mL.

Specifications

• Accession Number: P01375
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7124
• Molecular Weight (g/mol): 17 kDa
• Name: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Quantity: 1 mg
• Source: Human embryonic kidney cell, HEK293-derived human TNF-alpha protein Val77-Leu233
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.