R&D Systems™ Recombinant Mouse CD27 Ligand/TNFSF7 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to stimulate mouse T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1.25-5.0 μg/mL.

Specifications

• Accession Number: Q05A52
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 970
• Molecular Weight (g/mol): M.W. (Observed): 30 kDa, reducing conditions; M.W. (theoretical): 19.2 kDa
• Name: CD27 Ligand/TNFSF7
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived mouse CD27 Ligand/TNFSF7 protein MHHHHHHHHHH GGGSGGGSGGGS IEGR Mouse CD27 Ligand (Gln47 - Pro195) (N-terminus) Accession # Q05A52 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Cd70
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: CD27 Ligand/TNFSF7/CD70