R&D Systems™ Recombinant Mouse Frizzled-5 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Mouse Frizzled-5 Fc Chimera Protein is derived from NS0. The Recombinant Mouse Frizzled-5 Fc Chimera Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q9EQD0
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7855
• Molecular Weight (g/mol): Observed M.W.: 54-64kDa, reducing conditions, Theoretical M.W.: 43kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line,NS0-derived mouse Frizzled-5 protein, (N-terminus) Mouse Frizzled-5 (Ala27-Pro167) Accession &Num; Q9EQD0 IEGRMD Mouse IgG2a (Glu98-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: C2orf31, chromosome 2 open reading frame 31, DKFZP434E2135, frizzled (Drosophila) homolog 5, frizzled homolog 5 (Drosophila), frizzled-5, Frizzled5, Frz-5, FZ-5, FZD5, FzE5, HFZ5, MGC129692, seven-transmembrane receptor frizzled-5, Wnt receptor
• Common Name: Frizzled-5
• Gene Symbol: Fzd5
• Biological Activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is typically 10-60ng/mL in the presence of 5ng/mL of Recombinant Mouse Wnt-3a.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Mouse Frizzled-5 (Ala27-Pro167) Accession # Q9EQD0, N-terminus; IEGRMD; Mouse IgG2a (Glu98-Lys330), C-terminus
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining