R&D Systems™ Recombinant Mouse Hepsin Protein

Catalog No. 91-56S-E010

Supplier: R&D Systems™ 9156SE010

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >8,000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: O35453-2
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
• Gene ID (Entrez): 3249
• Molecular Weight (g/mol): M.W. (Observed): 41-47 kDa, reducing conditions; M.W. (theoretical): 42 kDa
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Hepsin protein Gln44-Pro416 (Asp160Glu & Arg161Lys), with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: EC 3.4.21, EC 3.4.21.106, hepsin, HPN, serine protease hepsin, serine protease hepsin catalytic chain, TMPRSS1 TMPRSS1serine protease hepsin non-catalytic chain, Transmembrane protease serine 1, transmembrane protease, serine 1
• Common Name: Hepsin
• Biological Activity: Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >8,000 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Gln44-Pro416 (Asp160Glu & Arg161Lys), with a C-terminal 10-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Hepsin