- Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
- ED50 for this effect is 0.06 to 0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4
|50 kDa (monomer)|
|>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.|
Mouse myeloma cell line, NS0-derived mouse Noggin protein
Mouse Noggin (Leu20-Cys232) Accession # U79163IEGRMDHuman IgG1 (Pro100-Lys330)N-terminus C-terminus
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.|
|Lyophilized from a 0.2 µm filtered solution in PBS and EDTA.|
|<0.10 EU per 1 µg of the protein by the LAL method.|
|NOG, noggin, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A|
|Reconstitute at 100 µg/mL in sterile PBS.|
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