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R&D Systems™ Recombinant S. pyogenes CRISPR-associated Protein 9
Purified CAS9 with Nuclear Localization Sequence
Supplier: R&D Systems™ 9957C9050
This item is not returnable.
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Description
Measured by its ability to cleave a targeted DNA substrate. S. pyogenes CRISPR-Cas9 achieves >80% substrate cleavage, as measured under the described conditions.Specifications
Enzyme Activity | |
901176 | |
50 μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. | |
<0.10 EU / 1 μg of the protein by the LAL method. | |
CRISPR-Cas9 | |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, Glycerol and TCEP. | |
M.W. (Observed): 133 kDa, reducing conditions; M.W. (theoretical): 164 kDa | |
E. coli-derived s. pyogenes CRISPR-Cas9 protein APKKKRKVGIHGVPAA S. pyogenes CRISPR-Cas9 (Asp2-Asp1368) (N-terminus) Accession # Q99ZW2 KRPAATKKAGQAKK-KKGYGRKKRRQRRRG HHHHHH (C-terminus) | |
RUO | |
Unconjugated | |
S. pyogenes | |
CRISPR-Cas9 |
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