R&D Systems™ Recombinant SARS-CoV-2 3CL Protease, CF

Description

E. coli Expressed, Bioactivity Tested

Recombinant SARS-CoV-2 3CL Protease is a cysteine protease that cleaves proteins with sequences including LQ(S/A/G). The cleavage occurs c-terminal to the glutamine residue. Reaction conditions will need to be optimized for each specific application. We recommend an initial SARS-CoV-2 3CL Protease concentration of 20-40 nM when using substrate S-720.

Specifications

• Accession Number: YP_009725301.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and EDTA.
• Gene ID (Entrez): 43740578
• Molecular Weight (g/mol): M.W. (theoretical): 34 kDa
• Quantity: 50 μg
• Source: E. coli-derived sars-cov-2 3 CL Protease protein Ser1 - Gln306
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: SARS-CoV-2 3 CL Protease