Invitrogen™ Red Firefly Luciferase Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1179

Catalog No. PIPA1179

Description

Western blot analysis of PA1-179 detects a prominent ~62 kDa protein in Red Firefly luciferase expressing cells. No signal was detected in Green Renilla transfected or untransfected control HEK293 cells. This antibody has also been successfully tested in IP, Immunofluorescence and neutralization assays. Red Firefly luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash or Glow Assay Kits or Luciferase Dual Assay Kits.

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light.

Specifications

• Antigen: Red Firefly Luciferase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; luciferin 4-monooxygenase; Photinus pyralis; photinus pyralis luciferase
• Immunogen: Purified full length recombinant Red Firefly luciferase expressed in E. coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunoprecipitation, Neutralization, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Luciferase
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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