Invitrogen™ RELM alpha Monoclonal Antibody (226033)

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MA523898

Catalog No. PIMA523898

Description

In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) RELM beta or rmResistin. Reconstitute at 0.5 mg/mL in sterile PBS.

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

Specifications

• Antigen: RELM alpha
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 5% trehalose and No Preservative
• Gene Accession No.: Q9EP95
• Gene Symbols: Retnla
• Immunogen: E. coli-derived recombinant mouse RELM alpha Asp24-Ser111.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Western Blot, Immunocytochemistry
• Clone: 226033
• Conjugate: Unconjugated
• Gene: Retnla
• Gene Alias: 1810019L16Rik; cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; Fizz1; Fizz-1; FIZZ2; found in inflammatory zone 1; Himf; HXCP2; Hypoxia-induced mitogenic factor; parasite-induced macrophage novel gene 1 protein; Pmng1; RELMa; RELMalpha; RELM-alpha; RELMb; RELMbeta; RELM-beta; resistin like alpha; resistin-like alpha; Retnla; Xcp2
• Host Species: Rat
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 57262
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized