RELM alpha Monoclonal Antibody (DS8RELM), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
12-544-180	LIFE TECHNOLOGIES 12544180	25 μg
12-544-182	LIFE TECHNOLOGIES 12544182	100 μg

Description

Description: This DS8RELM monoclonal antibody recognizes mouse RELM alpha, also known as FIZZ1. This antibody was shown not to cross-react with mouse RELM gamma. Applications Reported: This DS8RELM antibody has been reported for use in flow cytometric analysis. Applications Tested: This DS8RELM antibody has been tested by flow cytometric analysis of resident peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

Specifications

• Antigen: RELM alpha
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 1810019L16Rik; cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; Fizz1; Fizz-1; FIZZ2; found in inflammatory zone 1; Himf; HXCP2; Hypoxia-induced mitogenic factor; parasite-induced macrophage novel gene 1 protein; Pmng1; RELMa; RELMalpha; RELM-alpha; RELMb; RELMbeta; RELM-beta; resistin like alpha; resistin-like alpha; Retnla; Xcp2
• Gene Symbols: Retnla
• Isotype: IgG1 κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: DS8RELM
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q9EP95
• Gene ID (Entrez): 57262
• Immunogen: Recombinant mouse RELM alpha protein.
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Retnla