PeproTech RELM alpha Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No.	Quantity
500P214BT50	50 μg
500P214BT25	25 μg
50-271-8604	500 μg

Catalog No. 500P214BT50 Supplier PeproTech Supplier No. 500P214BT50UG

Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: MDETIEIIVE NKVKELLANP ANYPSTVTKT LSCTSVKTMN RWASCPAGMT ATGCACGFAC GSWEIQSGDT CNCLCLLVDW TTARCCQLS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMalpha. Anti-Murine RELMalpha-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine RELMalpha (500-P214) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RELMalpha. Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P214BT-1MG will be provided as 2 x 500 μg

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

Specifications

• Antigen: RELM alpha
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.1-1.0 mg/mL
• Conjugate: Biotin
• Formulation: PBS with no preservative
• Gene: Retnla
• Gene Accession No.: Q9EP95
• Gene Alias: 1810019L16Rik; cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; Fizz1; Fizz-1; FIZZ2; found in inflammatory zone 1; Himf; HXCP2; Hypoxia-induced mitogenic factor; parasite-induced macrophage novel gene 1 protein; Pmng1; RELMa; RELMalpha; RELM-alpha; RELMb; RELMbeta; RELM-beta; resistin like alpha; resistin-like alpha; Retnla; Xcp2
• Gene Symbols: Retnla
• Host Species: Rabbit
• Immunogen: E.coli-derived, 10.0 kDa Recombinant Murine RELMalpha.
• Purification Method: Antigen affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 57262
• Target Species: Mouse
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized