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Thermo Scientific™ MacConkey Sorbitol Agar with Cefixime and Tellurite
Description
Selectively detect sorbitol-negative Escherichia coli O157:H7 (VTEC) with MacConkey Sorbitol Agar with Cefixime and Tellurite.
Selectively detect sorbitol-negative Escherichia coli O157:H7 (VTEC) with Thermo Scientific™ Remel™ MacConkey Sorbitol Agar with Cefixime and Tellurite. The medium is recommended by Food and Drug Administration Center for Food Safety and Applied Nutrition (FDA/CFSAN) and the American Public Health Association (APHA) for selective isolation of VTEC from food samples6,7.
Verotoxin-producing Escherichia coli (VTEC), which includes serotype O157, is the fourth leading cause of diarrheal diseases in the United States4. This serotype of E. coli produces a verotoxin and is indistinguishable on the routine isolation media from those of non-VTEC E.coli. E. coli O157 is recognized as a cause of haemorrhagic colitis, an illness characterized by bloody diarrhoea and severe abdominal pain, and haemolytic uraemic syndrome (HUS)5 and as such, is a significant human pathogen.
Selectively detect sorbitol-negative Escherichia coli O157:H7 (VTEC) with MacConkey Sorbitol Agar with Cefixime and Tellurite.
- Ease of differentiation: Due to the presence of D-sorbitol; sorbitol negative strains form colorless colonies while other E. coli strains that ferment sorbitol and form pink colonies.
- Selective: Bile salts and crystal violet are selective agents which inhibit gram-positive organisms and enterococci, respectively. Cefixime and tellurite inhibit the growth of non-verotoxin producing E.coli and other non-sorbitol fermenting bacteria.
- Recommended as a simple, inexpensive, rapid and reliable means of screening for E. coli O157
- Recommended by FDA, Center for Food Safety and Applied Nutrition (FDA/CFSAN) and theAPHA for selective isolation of VTEC from food samples6,7.
In 1986, March and Ratnam introduced MacConkey Agar with sorbitol for the isolation of this serotype as unlike other serotypes VTEC does not ferment sorbitol1. In 1991, Chapman modified this medium by adding cefixime to specifically inhibit Proteus spp2. Zadik further modified it by adding potassium tellurite and found that it more effectively inhibited the growth of non-VTEC strains and increased isolation of E. coli O1573.
This medium contains peptones that provides nitrogenous compounds and amino acids essential for bacterial growth. Bile salts and crystal violet are selective agents which inhibit gram-positive organisms. Cefixime and tellurite inhibit the growth of non-verotoxin producing E.coli and other non-sorbitol fermenting bacteria. Lactose is substituted with D-sorbitol. Verotoxin- producing strains of E.coli ferment lactose but not sorbitol and form colorless colonies.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications
Specifications
Description | MacConkey Agar w/Sorbitol, Cefixime, Tellurite |
Format | 12mm x 85mm Monoplate |
Product Type | Agar |
Quantity | 10/Pk. |
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